91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

▍最近瀏覽歷史

[清空]

▍更多產(chǎn)品

貨號：B245025
名稱：Acanthamoeba healyi Moura et al.

貨號：B245024
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245023
名稱：Saccharomyces cerevisiae Meyen ex E.C. Hansen

貨號：B245022
名稱：Eutypella vitis (Schweinitz) Cooke emend. Glawe

貨號：B245021
名稱：Streptomyces scabiei (Thaxter) Lambert and Loria

貨號：B245020
名稱：Graphium cuneiferum (Berkeley et Broome) Mason et Ellis

貨號：B245019
名稱：HFBCI28

貨號：B245018
名稱：Septoria passiflorae Sydow, anamorph

貨號：B245017
名稱：Dictyostelium purpureum Olive

貨號：B245016
名稱：Y71

▍聯(lián)系我們

0574-87157013
mingzhoubio@163.com
浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
創(chuàng)e慧谷42號樓B幢401室

FHC

規(guī)格:

貨期:

編號:B164454

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產(chǎn)品名稱

FHC

商品貨號

B164454

Organism

Homo sapiens, human

Tissue

colon

Cell Type

Epithelial

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

13 weeks gestation

Storage Conditions

liquid nitrogen vapor phase

Images

Comments

When using a non-humidified incubator, gassing of cultures with 5% CO2 and plug-seal caps should be used to prevent evaporation of liquid in media and unintentional concentrating of media additives.

Although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected.

Complete Growth Medium

The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:

extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
Fetal Bovine Serum, 10% final conc (ATCC 30-2020)

The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM) 10 ng/ml cholera toxin 0.005 mg/ml insulin 0.005 mg/ml transferrin 100 ng/ml hydrocortisone fetal bovine serum 10%(final conc.
Subculturing	The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM) 10 ng/ml cholera toxin 0.005 mg/ml insulin 0.005 mg/ml transferrin 100 ng/ml hydrocortisone fetal bovine serum 10%(final conc.
Cryopreservation	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 4 to 6 X 10³ viable cell/cm² is recommended. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days Medium Renewal: Every 3 to 4 days.
Culture Conditions	Freeze Medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO Storage Temperature: Liquid nitrogen vapor phase
STR Profile	Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO₂), 5%
Name of Depositor	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,11 vWA: 16
Deposited As	DP Chopra
References	Homo sapiens

序號	文章標(biāo)題	操作
1	ATCC細(xì)胞運(yùn)輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購買須知	查看詳情
5	訂購流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479

利津县| 临邑县| 讷河市| 海城市| 静安区| 涪陵区| 忻城县| 宽甸| 宁晋县| 仁化县| 信宜市| 大邑县| 都江堰市| 伊春市| 澄江县| 建阳市| 盱眙县| 天等县| 筠连县| 文山县| 鄂伦春自治旗| 遵化市| 綦江县| 乌审旗| 南澳县| 梧州市| 洛浦县| 莲花县| 永和县| 徐州市| 弥渡县| 闸北区| 邛崃市| 定远县| 陕西省| 凤山市| 许昌县| 西贡区| 繁昌县| 始兴县| 西华县|