91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > BW5147.3(Thy-1-e).10
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
BW5147.3(Thy-1-e).10
BW5147.3(Thy-1-e).10
規(guī)格:
貨期:
編號:B164060
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BW5147.3(Thy-1-e).10
商品貨號 B164060
Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain AKR/J
Applications
This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47).
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are deficient in dolichol-P-mannose synthetase.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Derivation
This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47).
Comments
This line is a Class E Thy-1 negative (Thy-1-) mutant derived from BW5147.3 (ATCC TIB-47).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are deficient in dolichol-P-mannose synthetase.
The cells are resistant to 0.1 mM 6-thioguanine and 1 mM ouabain.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
Deposited As Mus musculus
References

Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934

Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. PubMed: 2880714

Chapman A, et al. The primary glycosylation defect in class E Thy-1-negative mutant mouse lymphoma cells is an inability to synthesize dolichol-P-mannose. J. Biol. Chem. 255: 4441-4446, 1980. PubMed: 7372584

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
马山县| 田东县| 锡林浩特市| 怀来县| 虞城县| 兴文县| 边坝县| 涟水县| 昭苏县| 宜兰县| 松原市| 梁山县| 武夷山市| 德惠市| 方城县| 万载县| 内江市| 贵州省| 武冈市| 盖州市| 宝山区| 海淀区| 建阳市| 金寨县| 东明县| 历史| 格尔木市| 关岭| 昌黎县| 永和县| 定边县| 乌拉特后旗| 农安县| 亳州市| 扎兰屯市| 河北区| 米泉市| 铁岭县| 县级市| 无棣县| 亳州市|