91精品国产综合成人,国产精品午夜自在在线精品,国内精品久久久久影院老司,国内精品久久久久久久久野战,国产精品美女久久福利网站,亚洲国产一成人久久精品,国产精品久久久久久一区二区三区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > BW5147.3
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
BW5147.3
BW5147.3
規(guī)格:
貨期:
編號(hào):B164059
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BW5147.3
商品貨號(hào) B164059
Organism Mus musculus, mouse
Tissue thymus
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease lymphoma
Strain AKR/J
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is a clone of the BW5147 cell line which was established from a spontaneous AKR/J thymoma.
Antigen Expression
H-2k; Thy-1.1
Comments

The cells are HAT resistant but sensitive to 0.001 mM cortisol and to 25 mcg/mL PHA.

Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: 5% CO2
Name of Depositor R Hyman, P Ralph
Deposited As Mus musculus
References

Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304

Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
九江市| 通州市| 泗阳县| 贵阳市| 嘉鱼县| 沁水县| 安岳县| 凯里市| 惠水县| 福鼎市| 大港区| 措美县| 民权县| 梨树县| 和龙市| 香格里拉县| 呼图壁县| 蒙山县| 河北区| 神农架林区| 元氏县| 石柱| 平度市| 奇台县| 柏乡县| 高雄县| 揭东县| 福海县| 都匀市| 嘉定区| 福鼎市| 黔江区| 大邑县| 西平县| 铜梁县| 兴义市| 于都县| 公安县| 巨鹿县| 栖霞市| 桂林市|