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240-13D10
240-13D10
規(guī)格:
貨期:
編號:B163647
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 240-13D10
商品貨號 B163647
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene).
Weaker activity against src and fgr was also detected.
In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes.
In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. Weaker activity against src and fgr was also detected.
In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines.
The IgG1 reactivity of the antibody with the v-yes peptide (778 to 793) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG1 and IgG2b
Name of Depositor National Cancer Institute
Deposited As mouse (B cell); mouse (myeloma)
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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